Fig. 6

CYR61 controls the expression level of IGF1Rβ and IGF1. a Expression pattern of IGF1Rβ protein in K7M2 and U2OS cell lines, as assessed by Western blot. Actin was used as loading control. b Expression pattern of IGF1 protein in K7M2 and U2OS cell lines, as assessed by ELISA. Results are expressed as mean ± standard deviation. a: p < 0.05 vs. control cells. c Relative luciferase activity of IGF1Rβ promoter construct in K7M2 and U2OS cell lines incubated in the presence or absence of recombinant CYR61. a: p < 0.05 vs. control cells; b: p < 0.05 vs. untreated. d Expression pattern of IGF1 in K7M2 and U2OS cell lines, as assessed by RT-qPCR. The relative mRNA levels were calculated using the 2^–ΔΔCT method and expressed as Log₂ of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells. e Expression pattern of a panel of mesenchymal and epithelial markers in CYR61 overexpressing cell lines, incubated with/without neutralizing anit-IGF antibody, as assessed by RT-qPCR. The relative mRNA levels were calculated using the 2^–ΔΔCT method and expressed as Log₂ of fold change (mean ± standard deviation). a: p < 0.05 vs. control cells