Table 1 Primers used in Real-time PCR
From: miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα
Name | Sequences |
|---|---|
hsa-miR-196a-5p | F: CCGACGTAGGTAGTTTCATGTT |
R: GTGCAGGGTCCGAGGTATTC | |
RT:GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACCCCAAC | |
hsa-U6 | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT | |
RT:GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACAAAATATGG | |
IκBα | F: CATCCTGAAGGCTACCAACTAC |
R: CATCAGCACCCAAGGACAC | |
β-actin | F: CTTAGTTGCGTTACACCCTTTCTTG |
R: CTGTCACCTTCACCGTTCCAGTTT |