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Fig. 6 | BMC Cancer

Fig. 6

From: Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinases

Fig. 6

Podoplanin silencing does not alter the expression of proteins involved in the epithelial-mesenchymal transition (EMT) in thyroid cancer cells. a. RT-qPCR analysis of mRNA levels in TPC1 and BcPAP thyroid cancer cells 48 h after transfection. The results were normalized to the 18S rRNA levels. The bars represent the average fold change in transcripts levels in cells transfected with siPDPN compared to cells transfected with siNeg. Values are means from at least three independent experiments. Data are presented as means ± standard errors of the mean (SEM) *P < 0.05; **P < 0.01. b. Western blot analysis of CD44, RhoA, Twist, Snail, Vimentin, PTK2 expression levels 48 h after transfection with siPDPN or control siNeg in TPC1 and BcPAP cells. Total protein were separated by SDS-PAGE, transferred onto a nitrocellulose membrane, and probed with specific antibodies. The results are representatives of three independent experiments

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BMC Cancer

ISSN: 1471-2407

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