Fig. 3From: Podoplanin (PDPN) affects the invasiveness of thyroid carcinoma cells by inducing ezrin, radixin and moesin (E/R/M) phosphorylation in association with matrix metalloproteinasesSoft agar (upper panel) and colony formation assay (lower panel). To analyze colony formation and anchorage-independent growth of TPC1 and BcPAP cells after PDPN silencing, the cells were seeded into 100 mm Petri dishes or 6-well culture plates containing soft agar. Colonies were stained with crystal violet and counted under a white light microscope at a 10x and 40x magnification. Data are presented as means ± standard errors of the mean (SEM) of results from at least three independent experiments, * < P, 0.05, .*** < P,0.001Back to article page