Fig. 1

Downregulation of podoplanin expression in BcPAP thyroid cancer cells following transfection with PDPN-specific siRNA. a. RT-qPCR analysis of PDPN mRNA levels in BcPAP cells 48 h after transfection with 30 nM siRNA specific for PDPN (siPDPN) or negative control siRNA (siNeg). The results were normalized to the 18S rRNA levels. The bars represent the average fold-change in PDPN transcript levels in cells transfected with siPDPN compared to control cells. The results are representative of four independent experiments. Data are presented as means ± standard errors of the mean (SEM), **: P < 0.01. b. Podoplanin protein levels in BcPAP cells 48 h after transfection with siPDPN or control siNeg by Western blot. β-actin was used as loading control c. Immunofluorescent staining of podoplanin in BcPAP cells transfected with siPDPN or control siNeg. Cells were stained with the anti-PDPN monoclonal antibody D2–40 followed by the DyLight549-conjugated secondary antibody (red), and counterstained with DAPI (blue). Magnification: 630x