Fig. 2

Increased levels of phospho-AMPKα in liver of 62-week-old mice, Hep3B cell, and Raw 264.7 cell. a Western blot analysis and quantification of phosphor-AMPKα in liver of 62-week-old mice. Beta actin was used as an internal control. b Western blot analysis and quantification of phosphor-AMPKα in Hep3B cell line. Beta actin was used as an internal control. Genistein was treated for 6 h after steoid starvation with charcoal dextran fetal bovine serum (CD-FBS) for overnight. c Western blot analysis and quantification of phospho-AMPKα in Raw 264.7 cell. Beta actin was used as an internal control. Genistein was treated for 6 h after steroid starvation with CD-FBS for overnight. Values represent means ± SEM of at least 3 experiments. *, P < 0.05. Numbers of mice used in experiment is at least each 6 for control and genistein groups