Fig. 2

Loss of SEMA5A increases migration ability in PC cells. a Images are showing scratch assay of Control and T3M-4-shSEMA5A at 0 h and 24 h (Scale bar represents 100 μm in length). b Quantitation of scratch assay using ImageJ software showing a significant (p = 0.004) enhancement of migration in T3M-4 SEMA5A knock-down cells. c Quantitation of scratch assay showing a significant (p = 0.05) enhancement of migration in CD18/HPAF SEMA5A knock-down cells. The bars in the graphs represent the mean percentage of the distance of migration ± SEM and the significance of the data is calculated using Student’s t-test. d Images are showing staining of the actin cytoskeleton in T3M-4-Control and T3M-4-shSEMA5A cells with the increased number of lamellipodium- and filopodium- like structures in SEMA5A knockdown cells. Images were taken using an LSM 710 Zeiss Confocal Microscope. Scale bar represents 10 μm in length. e A bar graph is representing quantitation of an average number of lamellipodia in T3M-4-Control and T3M-4-shSEMA5A cells showing an increase in the number of lamellipodia formed (p = 0.0079) in SEMA5A knock-down cells. f Bar graph depicting the quantitation of filopodia, showing an increase in the number of filopodia in T3M-4 SEMA5A knock-down cells. The values in the graph represent a number of Lamellipodia formation/ or filopodia per peripheral cell and the error bars ± SEM. The significance of the data is calculated using the non-parametric Mann-Whitney U test