Fig. 2

Ras-ERK1/2 repressed H3K9 acetylation to promote the growth and migration of SW48 cells. a SW48 cells were transfected with pEGFP-H3, pEGFP-K-Ras^G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras^G12V/T35S plus pEGFP-H3K9Q (with increasing amount 0.5, 1, and 2 g). a Transfection efficiency was tested by Western blot. b MTT assay was performed to assess cell viability. Subsequently, pEGFP-H3, pEGFP-K-Ras^G12V/T35S plus pEGFP-H3, or pEGFP-K-Ras^G12V/T35S plus pEGFP-H3K9Q (2 g) was transfected into cell, and c number of colonies and d cell migration were respectively determined by colony formation assay and transwell assay. ** P < 0.01; *** P < 0.001