Gene | Exon | Forward primer | Reverse primer | Previously reported mutations | Implicated in |
---|---|---|---|---|---|
BRAF | 11 | CTGTTTGGCTTGACTTGAC | CATATCCTATTATGACTTG | Minor mutation hotspots at codons 466 and 469 | Driver mutation, cutaneous melanoma |
15 | CTGATAGGAAAATGAGAT | CAGCAGCATCTCAGGGCC | p.V600E | ||
NRAS | 2 | GTGGCTCGCCAATTAAC | CCGACAAGTGAGAGAC | Mutation hotspots at codons 12 and 13 | Driver mutation, cutaneous melanoma |
3 | GCATTGCATTCCCTGTGG | GAACACAAAGATCATCC | p.Q61R, p.Q61L | ||
KRAS | 2 | GATAGTGTATTAACC | AACCTTTATCTGTATC | Mutation hotspots at codons 12 and 13 | Driver mutation,cutaneous melanoma |
3 | GTGCACTGTAATAATCT | AATTACTCCTTAATGTC | p.Q61R, p.Q61H | ||
GNAQ | 4 | CTTTCCGTAGACAGCTTTG | GTACTCAAGGCATAAAAG | p. R183Q | Driver mutation, uveal melanoma |
5 | GCTATATTTATGTTGAC | CTATCATTTACTTGTATC | p.Q209L, p.Q209R, p.Q209P | ||
GNA11 | 4 | GCAGCCGGCCTGAGCA | ACACACACTGAGGATG | p. R183C | Driver mutation, uveal melanoma |
5 | GCCAGGTGGCTGAGT | GCAGGGCCTTACTGG | p.Q209L, p.Q209P | ||
SF3B1 | 14 | GGCCGAGAGATCATTTCTAAT | AAGAAGGGCAATAAAGAAGGA | Mutation hotspot at codon 625, and multiple other hotspots | Prognosticating mutation in uveal melanoma, possible driver mutation |
EIF1AX | 1 | GCCACGCCTGCGTCATAAAG | CGAGCTCAGAGTCGCGTGTG | Mutation hotspots at codons 2,3,4,6 | Prognosticating mutation in uveal melanoma, possible driver mutation |
2 | AAAGGAAATTCCAAGAAGGGTAGGG | TAATCGTGCCACCACACTTCACC | Mutation hotspots at codons 13, 15 |