Fig. 5

Expressions of apoptosis and proliferation-related proteins in tumor tissues. a Left panel, representative fluorescence images for Ki-67 expression in tumor tissues. Ki-67 were stained in green, cell nuclei were counter stained with DAPI. Right panel, quantification analysis of Ki-67; the results are presented as the percentage of Ki-67 positive cells to the total number of cells in each image; b Left panel, representative fluorescence images for cleaved PARP expression in tumor tissues. Cleaved PARP expression were stained in red, and cell nuclei were counter stained with DAPI. Right panel, quantification analysis of cell apoptosis; the results are presented as the percentage of cleaved PARP positive cells to the total number of cells in each sample. All of the data represent mean ± standard deviation with n = 6. *: significant difference with p < 0.05. c and d Western blotting of p53, procaspase 9, acetyl histone H3 and γH2AX in delta-5-desaturase-WT and delta-5-desaturase-KD tumor tissues.The relative expression level of each protein was normalized using β-actin as a loading control; All of the data represent mean ± standard deviation with n = 6. *: significant difference vs. control with p < 0.05