Fig. 4

Promoted 8-hydroxyoctanoic acid inhibited growth of HCA-7 xenograft tumor. a Tumor size measurement of delta-5-desaturase-WT tumors during 4-week treatment of vehicle control, dihomo-γ-linolenic acid, 5-FU, or dihomo-γ-linolenic acid+ 5-FU. Insert: representative tumor photos from each treatment group at the end of the treatment; b Tumor size measurement of delta-5-desaturase-KD tumors during 4-week treatment. Insert: representative tumor photos from each treatment group at the end of the treatment; *: significant difference vs. corresponding control with p < 0.05; c Representative immunofluorescence images for delta-5-desaturase expression in tumor tissues; delta-5-desaturase was stained in pink, cell nuclei were counter stained with DAPI; d Quantification of the mean intensity of delta-5-desaturase staining in each sample. All of the data represent mean ± standard deviation with n = 6. *: significant difference with p < 0.05