Fig. 2

Dihomo-γ-linolenic acid treatment improves 5-FU’s efficacy in delta-5-desaturase-KD HCA-7 cells. a Colony formation assay of delta-5-desaturase-WT and delta-5-desaturase-KD HCA-7 cells after exposed to 100 μM dihomo-γ-linolenic acid and/or 50 μM 5-FU treatment for 48 h followed by additional 10 days incubation. *: significant difference with p < 0.05; b Annexin V-FITC/PI staining of delta-5-desaturase-WT and delta-5-desaturase-KD HCA-7 cells after 48 h incubation with 100 μM dihomo-γ-linolenic acid and/or 50 μM 5-FU. *: significant difference vs. control with p < 0.05, #: significant difference vs. 5-FU only group with p < 0.05. All of the data represent mean ± standard deviation with n ≥ 3