Fig. 1

Relationship between GRP78 and N-cad in MM.1S and PC3 cells upon GRP78 KD. 100 nM and 50 nM siRNA cocktail against GRP78 or a control siRNA were transfected into MM.1S and PC3 cells, respectively. Total mRNA and protein levels were analyzed at 48 h and 72 h, respectively. a Western blots of endogenous GRP78 and N-cad protein expression after GRP78 silencing. b Protein expression quantification. Target protein levels were normalized against the loading control GAPDH and compared with the control, non-targeting siRNA. Blot bands and quantitative values are presented as the mean ± SD and representative of 3 separate trials. Western blot analysis for MM.1S and PC3 cells were performed independently. *P < 0.05 and ***P < 0.001 in MM.1S cells and ****P < 0.0001 and **P < 0.01 in PC3 cells for GRP78 and N-cad, respectively. c qRT-PCR analysis of relative mRNA levels for GRP78 and N-cad upon GRP78 silencing. Target mRNA levels are relative to the control siRNA and represented as the mean fold change ± SD of 3 separate trials. *P < 0.05 in MM.1S cells and ***P < 0.001in PC3 cells for GRP78