Fig. 4

Propofol suppressed A549 cell proliferation and induced cell apoptosis by down-regulating miR-372. (a) The expression of miR-372 in A549 cells after miR-372 mimic or miR-372 inhibitor transfection was measured using qRT-PCR. After 8 μg/mL propofol treatment and/or miR-372 mimic (or miR-372 inhibitor) transfection, (b) the proliferation of A549 cells was detected using BrdU incorporation assay, (c) the protein expressions of p53, p16 and Cyclin D1 in A549 cells was assessed using western blotting, (d) the apoptosis of A549 cells was measured using Annexin V-FITC/PI staining and flow cytometry and (e) the protein expressions of Bcl-2, Bax, cleaved-Caspase 3 and cleaved-Caspase 9 in A549 cells were assessed using western blotting. N = 3.*P < 0.05, **P < 0.01, ***P < 0.001