Fig. 3

LXRα/β protein expression is correlated with resistance in ovarian cancer cells. a LXRα/β protein expression was measured by Western blot. (B1 and B2) Protein expression levels were quantitated by densitometry and normalized to GAPDH. The ratio of densities was calculated using PA-1 as control. The correlation between CDDP IC50 and LXRα/β protein expression, PAC IC50 and LXRα/β protein expression was determined by simple linear regression analysis. c Expression of LXRα/β protein after cholesterol (5 μg/ml) treatment measured by Western blot. d Expression of ABCG2, MDR1 and LXRα/β protein was measured by Western blot with cholesterol (5 μg/ml) treatment in ascites derived ovarian cancer cells. (E1 and E2) LXRα/β protein expression levels were measured by Western blot in primary cancer cells isolated from ovarian cancer patient derived ascites. Protein expression levels were quantitated by densitometry and normalized to GAPDH. The correlation coefficient square (R²) was determined by Pearson’s correlation coefficient test