Fig. 5
Performance of [18F]FDG as PET radiotracer for the evaluation of treatment with the oncolytic adenovirus VCN-01. (a) [18F]FDG PET images showing tumor evolution in the osteosarcoma model created with 531MII cell line. Images correspond to the non-treated positive control group (PBS control) and the two treatment groups with different adenovirus doses (virus 107 pfu and 108 pfu) studied at two different time points (PET 1 and PET 2). Red arrows point out tumors detected by PET (b and c). Box plot graphs for quantitative analysis of the [18F]FDG images, showing the evolution of SUVmax and TLG data between experimental groups over time and compared with negative controls without tumors (C-). $ symbol represent significant differences between data of the same group in the two time points ($ p < 0,05). * symbols represent statistically significant differences respect C- (* p < 0.05, ***p < 0,001). (d) Images of immunohistological analysis corresponding to the right hindlimbs of the same animals showed in (A), and obtained after the sacrifice at the end of the experiment (90 days). Serial sections were stained with hematoxylin and eosin and Ki-67, which detects cellular proliferation. Scale bars, 300 μm