Fig. 4

Survival of SW480 cells, but not of HCT116 cells depends on MTH1. a Knockdown efficiency was demonstrated by Western blotting. HCT116 and SW480 cells were harvested 72 h after knockdown induction. HIF-1β and β-actin served as control genes to confirm equal loading and protein transfer. b MTT assay in response to lentiviral knockdown of MTH1. Viability was measured 72 h after knockdown induction. c Apoptosis induction was assessed by measurement of caspase-3 activity 72 h after knockdown induction. d To calculate clonogenic survival, colonies were counted 10 days after knockdown induction. Bars were normalized to the control in each group. For statistical analysis Student’s t-test was used