Fig. 4

EMX2 restoration in U87 GB cells induces G1/S transition arrest. a-Sample clustering based on differentially expressed genes in Tet-induction (3916 DE probes). Pearson correlation distance is indicated on the right of the dendrogram. b-RT-qPCR was used to measure mRNA levels for genes associated with cell-cycle phases. Experiments were performed on the three EMX2 cl.B clones (EMX2 cl.B.1, EMX2 cl.B.2 and EMX2 cl.B.3). c-Western blotting experiment on EMX2 cl.A.1 and on control (empty vector) recombinant U87 cells using EMX2 and Cyclin B1 antibodies. VCP served as a loading control. Cells were grown in the absence (No Tet) or presence of tetracycline for 2 days (D2 Tet), 6 days (D6 Tet), 16 days (D16 Tet), and 8 days with following 8 days without tetracycline (D8 Tet) to test the induced phenotype and its reversibility. T-tests were performed using the No Tet condition as the reference and p-values were adjusted with BH correction. * p < 0.05, ** p < 0.01. d- Box plots representing mRNA expression normalized to levels obtained by microarray experiments for indicated probes of cell cycle-related genes. Transcriptome experiments were performed on the three EMX2 cl.A clones (EMX2 cl.A.1, EMX2 cl.A.2 and EMX2 cl.A.3). The differences between control conditions compared to No Tet were evaluated by t-test and corrected with BH. * p < 0.05, ** p < 0.01