Table 4 RNA-based analysis of equivocal ALK cases; 75% (6/8) of FISH-BL-positive cases were negative, 25% (2/8) were positive with both RNA-based methods. All FISH-BL-negative cases were ALK fusion negative with MPS and digital probe-based assay
From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance
Sample | IHC | FISH | Massive Parallel Sequencing (MPS) (IonTorrent) | Probe-Based Assays (NanoString) | Fusion | ||||
|---|---|---|---|---|---|---|---|---|---|
Fusion | 3′/5′ | Fusion | 3′/5′ | ||||||
IHC-uncertain/FISH-BL-positive | |||||||||
 P19 | ± | + | – | – | – | – |  | ||
IHC-positive/FISH-BL-positive | |||||||||
 P20 | + | + | + | + | ± | ± | + | + | V3a/b |
 P21 | + | + | + | + | + | + | V1 | ||
IHC-negative/FISH-BL-positive | |||||||||
 P22 | – | + | – | – | – | – |  | ||
 P23 | – | + | – | NA | – | NA | – | – |  |
 P24 | – | + | – | – | – | ± |  | ||
 P25 | – | + | – | – | – | – |  | ||
 P26 | – | + | – | – | – | – |  | ||
IHC-negative/FISH-BL-negative | |||||||||
 P27 | – | – | – | – | – | – | – | – |  |
 P28 | – | – | – | – | – | – |  | ||
 P29 | – | – | – | – | – | – |  | ||
 P30 | – | – | – | – | – | – |  | ||
IHC-negative/FISH-positive | |||||||||
 P31 | – | + | – | – | – | – |  | ||
 P32 | – | + | – | – | – | – |  | ||
 P33 | – | + | – | – | – | – |  | ||