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Table 4 RNA-based analysis of equivocal ALK cases; 75% (6/8) of FISH-BL-positive cases were negative, 25% (2/8) were positive with both RNA-based methods. All FISH-BL-negative cases were ALK fusion negative with MPS and digital probe-based assay

From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance

Sample IHC FISH Massive Parallel Sequencing (MPS) (IonTorrent) Probe-Based Assays (NanoString) Fusion
Fusion 3′/5′ Fusion 3′/5′
IHC-uncertain/FISH-BL-positive
 P19 ± +  
IHC-positive/FISH-BL-positive
 P20 + + + + ± ± + + V3a/b
 P21 + + + + + + V1
IHC-negative/FISH-BL-positive
 P22 +  
 P23 + NA NA  
 P24 + ±  
 P25 +  
 P26 +  
IHC-negative/FISH-BL-negative
 P27  
 P28  
 P29  
 P30  
IHC-negative/FISH-positive
 P31 +  
 P32 +  
 P33 +  
  1. Fusion: fusion detection with amplicons/probes covering ALK fusion breakpoint; 3′/5′: fusion detection with amplicons/probes covering 3′ and 5′ end; NA: Not Analyzable; ND: Not Determined; +: fusion detected; −: fusion not detected; ±: fusion uncertain; V1: EML4_13:ALK_20; V3a: EML4_6a:ALK_20; V3b: EML4_6b:ALK_20