From: RNA-based analysis of ALK fusions in non-small cell lung cancer cases showing IHC/FISH discordance
Sample | IHC | FISH | Massive Parallel Sequencing (MPS) (IonTorrent) | Probe-Based Assay (NanoString) | Fusion | ||||
---|---|---|---|---|---|---|---|---|---|
Fusion | 3′/5’ | Fusion | 3′/5’ | ||||||
IHC-negative/FISH-negative | |||||||||
 P01 | – | – | NA | NA | NA | NA | ND |  | |
 P02 | – | – | – | – | ± | – | – | – |  |
 P03 | – | – | – | – | ± | – | – | – |  |
 P04 | – | – | – | – | – | – |  | ||
 P05 | – | – | – | – | – | – |  | ||
 P06 | – | – | – | – | ND |  | |||
 P07 | – | – | – | – | ND |  | |||
 P08 | – | – | – | – | ND |  | |||
 P09 | – | – | – | – | ND |  | |||
 P10 | – | – | – | – | ND |  | |||
 P11 | – | – | – | – | ND |  | |||
 P12 | – | – | – | – | ND |  | |||
IHC-positive/FISH-positive | |||||||||
 P13 | + | + | + | + | + | – | + | + | V1 |
 P14 | + | + | + | ± | + | + | V1 | ||
 P15 | + | + | + | + | + | + | V3a/b | ||
 P16 | + | + | + | + | + | + | V2 | ||
 P17 | + | + | + | + | ND | V1 | |||
 P18 | + | + | + | + | + | + | ND | V6 |