Fig. 1
From: A tight balance of Karyopherin β1 expression is required in cervical cancer cells
Kpnβ1 localizes to the nucleus and nuclear membrane in cervical cancer cells and enhances the nuclear import of known Kpnβ1 cargoes. a Immunofluorescence analysis was used to determine GFP expression across the HeLa and CaSki EGFP and Kpnβ1-EGFP stable cell lines. Representative GFP-Alexa 488 images are shown for each cell line. Enlarged images in the top right-hand corner show perinuclear localization in representative Kpnβ1-EGFP cells. b Western blot analysis was used to determine endogenous Kpnβ1 (97 kDa) and exogenous Kpnβ1-EGFP (129.7 kDa) expression levels across the HeLa and CaSki EGFP and Kpnβ1-EGFP cell lines. The first lane represents protein harvested from untransfected cells. GAPDH was used as a control for loading. c - f Stable expression of Kpnβ1-EGFP in HeLa cells resulted in a significant increase in NFAT (c), AP-1 (d), PMA-stimulated NFκB p65 (e) and p53 (f) promoter-driven luciferase activity. Results shown represent the mean ± SEM (*p < 0.05)