Fig. 6

Effects of ATRA on EMT in MCF10DCIS cells. a Representative Western blot analysis with the indicated antibodies of lysates from MCF10DCIS cells grown at normoxia (N) or hypoxia (H) for 96 h in the presence or absence of 1 μM ATRA. b Relative levels of E-cadherin, β-catenin and Vimentin as deduced from the densitometry of immunochemical bands normalized with β-Tubulin, used as internal control for equivalence of loaded proteins. c Representative fluorescence microscopy images of MCF10DCIS cells grown on glass dishes for 96 h at normoxia or hypoxia in the presence or absence of 1 μM ATRA and subjected to immunocytochemical analysis with the anti-β-catenin antibody. Bar: 20 μm. d Representative immunochemical analysis performed with the indicated antibodies of lysates from MCF10DCIS cells grown at normoxia (N) or hypoxia (H) for 96 h in the presence or absence of 1 μM ATRA. e Relative levels of SLUG as deduced from the densitometry of immunochemical bands normalized with β-Tubulin, used as internal control for equivalence of loaded proteins. Immunoblots shown have been cropped to conserve space. All the data are the mean of three separate experiments performed in triplicate ±SD. *P < 0.05 between bars; #P < 0.05 versus normoxia taken as 1