Fig. 1

Effects of low oxygen availability on MCF10DCIS cells. a Growth of MCF10DCIS cells cultured under low oxygen for the indicated times. The data show the number of viable cells relative to normoxia, taken as 1. b Representative Western blot analysis with the indicated antibodies of lysates from MCF10DCIS cells grown at hypoxia for the indicated times. Immunoblots shown have been cropped to conserve space. c Levels of HIF-1α and CAIX as deduced from the densitometry of immunochemical bands normalized with β-Tubulin, used as internal control for equivalence of loaded proteins. All the data are the mean of three separate experiments performed in triplicate ±SD. #P < 0.05 with respect to normoxia