Fig. 3

Reduction of OGT inhibited the growth of bladder cancer cells in vitro and xenograft tumor growth in vivo. (a) The expression of OGT and O-GlcNAcylation after infection with LV-sh-OGT or LV-sh-NC. MTT assay (b) and cell colony formation assay (c) were used to determine the proliferation of T24 and UMUC-3 cells after transfection with LV-sh-OGT and LV-sh-NC. Following infection with LV-sh-OGT or LV-sh-NC, UMUC-3 cells were implanted subcutaneously in 6-week-old nude mice. The tumor growth was evaluated following 3 weeks of tumor implantation. Representative images (d) and weight (e) of the excised tumors derived from nude mice are shown. (f) Representative images of hematoxylin and eosin staining and Ki-67 immunohistochemical detection of the excised tumors derived from nude mice. Data are shown as mean ± standard deviation of three independent experiments. *P < 0.05