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Fig. 3 | BMC Cancer

Fig. 3

From: APC gene is modulated by hsa-miR-135b-5p in both diffuse and intestinal gastric cancer subtypes

Fig. 3

Western Blot of DNMT3A, CDC42 (a) and APC (b) proteins in control and transfected cells of AGP01, ACP02 and ACP03 cell lines. (a) DNMT3A and CDC42 were down-regulated after the transfection of mimics of hsa-miR-29c-5p in AGP01 and ACP03 2D cell lines. There was no difference in the expression of both proteins in transfected cells of ACP02 2D cell line. b APC was up-regulated in all three 2D cell lines after the transfection of hsa-miR-135b-5p antimiRs. β-Actin was used as a loading control in all blot experiments. NC stands for Negative Control; M for transfected with mimics; and Am for transfected with antimiRs

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