Fig. 8

PKD2 knockdown inhibited bombesin-stimulated proliferation of HNSCC cells. a PKD2 depletion suppressed GRP-induced ERK1/2 activation. 686LN cells were transfected with a PKD2-targeting siRNA (si-D2–2) and a non-targeting siRNA (si-NT). Two days after transfection, cells were harvested and analyzed by immunoblotting for the indicated proteins. Representative images from one of four independent experiments are shown. b Knockdown of PKD2 blocked bombesin-induced proliferation of 686LN cells. 686LN cells transfected with si-NT and si-D2–2 were grown in the absence or presence of bombesin (20, 200 nM) for 3 days. Cell proliferation was measured by MTT assay. Representative data from one of three independent experiments are shown. ** P < 0.01, ****P < 0.0001