Fig. 1

Sorafenib reverses DHA-induced suppression of nuclear Bach1 protein expression and induction of HO-1 transcription in A2780 cells. a Cells were pretreated with Sorafenib for 1 h prior to addition of DHA for 18 h. Nuclear proteins were isolated and Bach1 expression was determined by Western blot (n = 3). b Cells were pretreated with Sorafenib for 1 h prior to addition of DHA for 24 h. Cell lysates were prepared and HO-1 expression was determined by Western blot (n = 3). c Cells were transfected with the pGL3/4.5-HO-1 luciferase reporter construct and pretreated with Sorafenib for 1 h prior to addition of DHA for 24 h. Luciferase activity was determined and expressed relative to untreated controls (n = 3, mean ± SEM, *p < 0.01)