Fig. 4

ACTL6A promoted epithelial mesenchymal transition of colon cancer cells. a-b Real-time PCR (a) and western-blot results (b) showed that the expression level of the mesenchymal marker vimentin and snail were upregulated when ACTL6A was overexpressed but was downregulated when ACTL6A was knocked down; and vice versa, epithelial marker E-cadherin was upregulated when ACTL6A was knocked down but was downregulated when ACTL6A was overexpressed. c Analysis of the cell morphology showed that SW480 cells were more elongated when ACTL6A was overexpressed, while SW620 cells changed to an oval shape when ACTL6A was knocked down. d Cell immunofluorescence assay showed that ACTL6A knockdown induced a stronger E-cadherin and weaker Vimentin expression in SW620 cells, while ACTL6A overexpression in SW480 cells had an opposite effect