Fig. 2

Uc.416 + A is involved in cell proliferation and migration in 786-O cells. a The results of qRT-PCR analysis for the expression of Uc.416 + A in 786-O and Caki-1 cells. The results are expressed as the mean ± S.D. of triplicate measurements. b The results of qRT-PCR analysis for the expression of Uc.416 + A in 786-O cells transfected with negative control or two different siRNAs. The results are expressed as the mean ± S.D. of triplicate measurements. ***P < 0.001. c Cell proliferation assay in 786-O cells transfected with negative control or two different siRNAs. Cell growth was assessed by MTT assays at 1, 2, and 4 days after seeding on 96-well plates. Bars and error bars indicate the mean and S.D., respectively, of 3 independent experiments. **P < 0.01. d Wound healing assay in 786-O cells transfected with negative control or two different siRNAs. Wound closures were evaluated by wound contraction percentage and closure time at 0, 12, and 24 h after scratching. The results are expressed as the mean and S.D. of triplicate measurements. **P < 0.01