Fig. 5
From: PD-L1/PD-1 crosstalk in colorectal cancer: are we targeting the right cells?
Proliferative properties of T-lymphocytes cocultured with monocytes/macrophages and colorectal cancer stem cells. Expression of PD-1 by CD4+ (a) and CD8+ (b) populations within naïve lymphocytes (φ, white boxes) vs. lymphocytes cocultured (T, grey boxes) for 5 days with isolated tumour cells from colorectal tumour samples and monocytes/macrophages. Representative gating strategy to analyse the proliferation of CD4+ T-lymphocytes (c) and their proliferation in the presence (+) or not (−) of an α-PD-1 antibody, as measured by CFSE dimming (d). * p < .05, ** p < .01 using a Wilcoxon test