Fig. 3

a Percentage apoptosis induction following 72 h treatment with increasing concentrations of TRAIL in SKBR3-Par and SKBR3-L cells. ‘*’ indicates a significant increase (p < 0.05 as calculated by Student’s t-test) in TRAIL induced apoptosis between SKBR3-Par and SKBR3-L cells at the relevant concentration. b Western blot analysis of PARP cleavage relative to total PARP following treatment with 25 ng/mL TRAIL for 6, 24 and 48 h in SKBR3-Par and –L cells. c The effect of 8-h of TRAIL (100 ng/ml) treatment on caspase 3/7 activity normalised to cell viability relative to untreated controls in SKBR3-Par and SKBR3-L cells. Error bars represent the standard deviation of triplicate independent experiments. ‘*’ indicates a significant increase (p < 0.05 as calculated by Student’s t-test) in Caspe3/7 induction by TRAIL in SKBR3-L cells relative to SKBR3-Par cells