Fig. 7

Mirin inhibition of MRE11 reduces androgen-stimulated gene expression. a Mirin dose response of androgen-mediated transcription (2 nM R1881, 12 h). Relative expression (RT-qPCR) of FKBP5 mRNA in LNCaP, VCaP, and PC3-AR cells. b Relative expression (RT-qPCR) of SOCS2, HOMER2, and ABCC4 in PC3-AR cells. c Relative expression (RT-qPCR) of three androgen-mediated genes PSA, FKBP5, and TMPRSS2 after inhibiting MRE11 with mirin or ATM with KU55933 in LNCaP cells. d Alamar blue cell viability assay measuring mirin effect on cell growth/survival in RWPE-1, LNCaP, VCaP, and PC3-AR after 72 h. e Mirin IC₅₀ values derived from Alamar blue cell viability assays from three experimental replicates