Fig. 4

Reduced expression of multiple DNA repair genes occurs in response to androgen treatment. a Heatmap of DNA damage repair genes in prostate cancer cell line models. RNA-sequencing from untreated and androgen-treated cells were Z-score normalized for the 32 DNA damage repair genes reported by Polkinghorn et al. (2013) [50] and 28 control AR-target genes reported by Hieronymus et al. (2006) [52]. Genes in bold indicate differential regulation across all three cell lines. b Heatmap of DESeq2 log2 fold changes of DNA damage repair genes in prostate cancer cell line models. c GSEA of the RNA-seq data from prostate cancer lines, limited to the 32- DNA repair gene set signature