Fig. 6

Knockdown of ANRIL mediates NF-κB and Wnt/β-catenin signaling pathways by regulation of miR-191. The expression vectors of sh-ANRIL#1, sh-ANRIL#2 and miR-191 mimic were transfected into HepG2 cells to alter ANRIL and miR-191 expression. Western blot assay was used for determining the protein levels of (a and b) p/t-p65 and p/t-IκBα and (c and d) β-catenin in these transfected cells. ANRIL: CDKN2B antisense RNA 1; miR-191: microRNA-191; NF-κB: nuclear factor-kappa B; *P < 0.05; ***P < 0.001