Fig. 5

The tumour suppressive function of TUSC2P is dependent on miRNA binding. TUSC2P 3’ UTR or empty vector was transfected in parental Ec109 or Ec109 DICER−/− cells. a De-repression of TUSC2 abundance by TUSC2P 3’ UTR over-expression was blunted in DICER −/− EC109 cells. b-d Effects of TUSC2P 3’UTR over expression on growth (b), invasion (c) and apoptosis (d) were also blunted in DICER −/− EC109 cells (n = 3)