Decreased methylation in the SNAI2 and ADAM23 genes associated with de-differentiation and haematogenous dissemination in breast cancers

Table 2 Primers for PCR and pyrosequencing

Gene	Orienta-tion	Sequence (5′-3′) of PCR primer	PCR product (bp)	Sequence (5′-3′) of pyrosequencing primer	Numb. of CpGs
ADAM23 ^a	Forward Reverse	[Biotin]GCGTCGTTTTAGTATTTTTAGGTT TCCCCAACCACTACTCCCT	89	ACTACTCCCTCCCCC	8
uPA	Forward Reverse	TAGGTGTATGGGAGGAAGTA [Biotin]CTCCCTCCCCTATCTTACA	165	GTTTTTTTTAAATTTTTGTGAG	7
CXCL12 ^b	Forward Reverse	TAGTGGGGTTTTGTTATAGGGATA [Biotin]ACCTTTAACCTTCTCAAACTC	121	GGGTTTTGTTATAGGGATAAT	7
TWIST1	Forward Reverse	GAAGGGGAGGGAAGGGG [Biotin]TAACAATTCCTCCTCCCA	97	GGGAGGGAAGGGGGAG	3
SNAI1	Forward Reverse	GTATTTGTTAGGGGAGTGGT [Biotin]ACCACCCCCCTTTATCAC	72	GTTAGGGGAGTGGTTT	6
SNAI2	Forward Reverse	[Biotin]GTTGGTTTGGTGTGGTGTAG CCCTACCCCCCTAACTTCCAAATATAAT	100	CCTAACTTCCAAATATAATACAAC	5

^a,bSet of PCR primers and pyrosequencing primers were previously published by Zmetakova and colleagues [54]

ISSN: 1471-2407