Fig. 3
From: Association of tamoxifen resistance and lipid reprogramming in breast cancer
Free cholesterol accumulates in lysosomes in the resistant cells. Intracellular accumulation of free cholesterol (blue) accumulates in lysosomes (orange) stained with two lysosomal markers, Lamp1 (a) and Lamp2 (b), detecting the lysosomal-associated-membrane-proteins 1 and 2 +/āā1Ā Ī¼M 4-OH-tamoxifen. Tamoxifen-resistant cells are less sensitive to lysosomal membrane permeabilization detected with galectin-3 (orange) translocation (c, images were differently enhanced for visualization purposes) and measurement of galectin-3 positive cells (d upper graph) as well as number of galectin-spots per cell (d lower image). Galectin-3 measurements were done on the raw image, nā=ā4 for each condition. Only significant differences (p-value <ā0.05) between the same clone as well as of the comparison between resistant and tamoxifen-resistant cells in the same treatment conditions are indicated, all other comparisons can be found in Additional fileĀ 3. e Mature cathepsin D is downregulated in tamoxifen resistant cells