Fig. 2

a Representative histology (H.E. staining) of the mesenteric WAT of KC;Hsl^+/+ and KC;Hsl^−/− mice. Scale bar represents 100 μm. Quantification of crown-like structures (CLS) in KC;Hsl^+/+ and KC;Hsl^−/− mice (right). *: p < 0.01. b Immunofluorescence staining of mesenteric WAT of KC;Hsl^+/+ and KC;Hsl^−/− mice (two representative mice in each group). Red and green staining denote F4/80 (macrophage marker) and TNF-α (as a marker of tissue inflammation), respectively. Scale bar represents 100 μm. Semi-quantitative analysis of TNF-α expressing macrophages (orange staining) in KC;Hsl^+/+ and KC;Hsl^−/− mice (table below). c Quantification of PL, TG, and FFA content in mesenteric WAT of KC;Hsl^+/+ and KC;Hsl^−/− mice (n = 5). Western blot analysis of HSL and PPAR-γ (total ERK as loading control) in KC;Hsl^+/+ and KC;Hsl^−/− mice (lower left). d Changes (≥ 50% increase or decrease compared to KC;Hsl^+/+) in several cytokines and chemokines in the mesenteric WAT of KC;Hsl^−/− mice as detected by a Mouse Cytokine Antibody Array (n = 3 biological replicates). Representative array blots (Cytokine Array 3 and 4) in KC;Hsl^+/+ and KC;Hsl^−/− mice (right)