Fig. 6

Knockdown of GNB4 inhibits proliferation and induces cell cycle arrest and apoptosis. a and b, 182^R-6 and TAM^R-1 cells grown to 80% confluency were transiently transfected with either 30 nM GNB4 siRNA or 30 nM negative control siRNA; 24 h after transfection, the cells were replated in 96-well plate; the MTT assay was performed as described in “Methods”; 72 h (TAM^R-1) or 96 h (182^R-6) after transfection, the cells were harvested for cell cycle and apoptosis analyses. Asterisk indicates p < 0.05