Fig. 3

The ectopic expression of GNB4 enhances the proliferation of antiestrogen-resistant breast cancer cells. a, 182^R-6 and TAM^R-1 cells were transfected with either pEGFP-GNB4 or pEGFP-C1; after G418 selection, whole cellular lysate prepared from the positive cells was subjected to Western blot analysis using an antibody against GNB4. b and c, MTT assay was performed using 182^R-6 (b) and TAM^R-1 (c) cells stably expressing GNB4 or GFP as described in “Methods”. Asterisk indicates p < 0.05