Fig. 8
From: Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines
NCO-90/141 induce autophagy. S1T, MT-2, and Jurkat cells were treated with NCO-90/141 and STF-62247 (50 μM), an autophagy inducer, for 48 h. a Autophagy was measured by quantifying the mean fluorescence intensity using a Cyto-ID Autophagy Detection Kit. b Protein levels were detected by western blotting with the indicated antibodies. c Cellular autophagic flux monitored by lapidated LC3-II was evaluated using the FlowCellect™ Autophagy LC3 Antibody-based Assay Kit. S1T, MT-2 Jurkat, and HL60 cells were treated with 50 μM NCO-90 (upper) or NCO-141 (lower) for 24 h