Fig. 5
From: Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines
NCO-90/141 induce caspase-independent cell death. S1T, MT-2, Jurkat, and HL60 cells were treated with NCO-90 (50 μM) or NCO-141 (S1T and MT-2 cells, 50 μM; Jurkat and HL-60 cells, 100 μM) and Z-VAD-FMK (40 μM) for 72 h. a Viability of cultured cells was measured by Cell Count Reagent SF. b and c Annexin V-, 7-amino-actinomycin D (7AAD)-, and TUNEL-positive cells were detected by flow cytometry. Data represent mean percentages ± SD of three independent experiments