Fig. 3

Expressions of β-catenin and Wnt components. Hepa1–6 cells were grown for 7 days in complete media (C) or serum-free media (SF); a Western blot analysis of cytoplasmic and nuclear β-catenin (upper panel), densitometry image analysis (middle panel), and qRT-PCR analysis of total RNA (lower panel); b Western blot analysis of total protein for downstream targets of canonical Wnt/β-catenin signaling and analysis of GSK-3β Ser-9 phosphorylation status. All blots from the same experiment, and normalized to β-actin. *(p ≤ 0.05), **(p ≤ 0.005), n = 3 independent experiments. Cyto = Cytoplasm; Nuc = Nuclear; C=Control; SF=Serum-free