Fig. 5

LASP1 regulated pivotal biologic process by activating PI3K/AKT pathway. a Glioblastoma data in TCGA database was analyzed by using cBioPortal tools (http://www.cbioportal.org/index.do). Each blue dot indicates one case of glioblastoma. Phosphorylated AKT3 at Ser473 is positively correlated with LASP1 mRNA level, while the total level of AKT3 has no significant correlation with LASP1; (b) Western blotting analysis of p-PI3K, PI3K, p-AKT(Ser473), AKT proteins in indicated cells transfected with si-LASP1; (c) LY294002 inhibited tumor growth and dramatically accelerated the suppression effect of TMZ in U87 and U251 cell lines. ^* P < 0.05, ^** P < 0.01. ^***p < 0.001