Fig. 4

Quantification of DNA damage using γ-H2AX foci numbers. All the cells were collected at 0 h, 0.5 h, 1 h, 2 h, 4 h, 6 h and 8 h after irradiation with 8 Gy X-rays. DNA damage was evaluated as γ-H2AX foci numbers. a Nm23-H1-low-expressing group. b Nm23-H1-over expressing group. c The γ-H2AX foci image of both groups. The γ-H2AX protein is stained with red fluorescent and the nucleus is stained with blue fluorescent. 1. A549-shNm23-H1; 2. DOX+ A549-shNm23-H1; 3. A549-nNm23-H1; 4. A549-vector. (*: p < 0.05; **: p < 0.01)