Fig. 4

EZH2 overexpression in normal breast epithelial cells affects the expression of orphan nuclear receptors. a, Western blot shows the alteration in the expression of ERRs upon ectopic expression of EZH2 in (i), MCF-7 cells and in normal breast epithelial (ii) MCF10A cells. b, Transcript level of ERRs in (i) MCF-7 and (ii) MDA-MB-231 upon transfection of cDNA construct of EZH2 (iii). Graphs were plotted with SD, which is calculated from three independent experiments. One-way ANOVA was used for statistical analysis for experiments done in triplicate. ** P < 0.005, *** P < 0.001