Fig. 5
From: Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines
Re-sensitization of SK-MEL-28PLXr and A2058PLXr cells to PLX treatment by p-ERK inhibitor PD98059. a SK-MEL-28PLXr cells and A2058PLXr cells were treated by PD98059 and PLX for 72 h as indicated. Cell viability assay was performed by MTT. Results are expressed as the percent of viable cells, and values represent the means ± SD (bars) of three independent experiments. b A2058 PLXr cells and SK-MEL-28 PLXr cells were seeded in 48-well plates. Cells were treated by PD98059 as indicated. Cells were transfected with either ABCB5 control siRNA or ABCB5 siRNA the next day. After 6 h of transfection, PLX was added to each well as designated. Cell viability assay was performed by MTT after 72 h of transfection. Results are expressed as the percent of viable cells, and values represent the means ± SD (bars) of three independent experiments (*p < 0.05, ** p < 0.01, n = 3)