Fig. 3
From: Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines
Differential expression of ABCB5, ERK, Akt, p-ERK, and p-Akt in TMZ-treated and PLX-treated melanoma cells. a Total RNA from TMZ-treated and PLX-treated melanoma cells were isolated. Real-time PCR was performed as described in Materials and Methods. B2M was used as an internal control. The bar graphs show expressed fold changes of PLX-resistant cell lines relative to each of their parent cell lines adjusted for B2M. Each of the experiments is a representation of two independent experiments performed in triplicate (** p < 0.01, n = 3). TMZ P5: Passage 5 of TMZ-treated melanoma cells. PLX P5: Passage 5 of PLX-treated melanoma cells. b Differential expression of ABCB5, ERK, Akt, p-ERK, and p-Akt in early passages of TMZ-treated and PLX-treated cells by Western blot analysis. Targeted protein/actin showed the ratio of the scanned optical density of each protein band. TMZ P5: Passage 5 of TMZ-treated melanoma cells. PLX P5: Passage 5 of PLX-treated melanoma cells