Fig. 4

Inhibition of nuclear export causes loss in the cytoplasm and accumulation in the nucleus of TET2. a IHC analysis of TET2 after treatment of the SI-NET cell lines CNDT2.5 (adhesive cells) and KRJ-I (suspension cells) with the exportin-1 (XPO1/CRM1) inhibitor leptomycin B. Scale bar, 50 μm. Nuclear accumulation of TET2 is seen. Small labeled dots in the treated KRJ-I cells constitute cellular debris. b Western blot analysis of cytoplasmic and nuclear protein extracts from CNDT2.5 and KRJ-I cells after leptomycin B treatment. Induced absence in the cytoplasm and nuclear retention of TET2 is seen. β-tubulin and lamin A/C was used as marker for the cytoplasm and nucleus, respectively. c Real-time RT-PCR of XPO1 in paired primary SI-NETs (n = 19) and metastases (n = 22). Normal tissues from the small intestine (n = 3) was arbitrarily used for comparison. A representative overall positively XPO1 stained metastatic tumor is shown to the right. Scale bar, 100 μm