Fig. 2

IHC analysis of 5hmC, TET1, and TET2. Representative results are shown. Scale bar, 50 μm. a: A primary SI-NET shows a large area with negatively stained cell nuclei for; (1) 5hmC, (2) TET1, (3) TET2. b: A metastatic tumor shows mosaic pattern of staining, i.e. a mixture of positive and negative cell nuclei, regardless of cell number, staining strength for; (1) 5hmC, (2) TET1, (3) TET2 with Cytoplasmic staining. c: Two primary SI-NETs with (1) the mosaic TET2 cytoplasmic staining together with the presence of negative nuclei, (2) the cytoplasmic staining together with the mosaic nuclear staining patterns, (3) Negative staining was observed only without the primary antibody. d: Real-time RT-PCR of TET1 and TET2 reveals no difference in expression between paired primary tumors (n = 19) and metastases (n = 22)